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Gating strategy for cell sorting of osteomacs (CD45 + ALP − <t>CD14</t> + ). After Gating of single cells, using the width of the forward and side-scatter signal (FSC-W, SSC-W), living cells were selected (Zombie-Aqua-). CD45 + ALP − cells were then selected to identify the osteomac population by CD14 + . Those cells were selected for sorting. Shown here is a representative FACS run. This FACS protocol was established with n = 3 donors.
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Gating strategy for cell sorting of osteomacs (CD45 + ALP − <t>CD14</t> + ). After Gating of single cells, using the width of the forward and side-scatter signal (FSC-W, SSC-W), living cells were selected (Zombie-Aqua-). CD45 + ALP − cells were then selected to identify the osteomac population by CD14 + . Those cells were selected for sorting. Shown here is a representative FACS run. This FACS protocol was established with n = 3 donors.
369 Miltenyi Biotec Pe Apc Cd14 M5e2 Mouse Igg2a Kappa Nb10077758b Novus Biologicals, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Gating strategy for cell sorting of osteomacs (CD45 + ALP − <t>CD14</t> + ). After Gating of single cells, using the width of the forward and side-scatter signal (FSC-W, SSC-W), living cells were selected (Zombie-Aqua-). CD45 + ALP − cells were then selected to identify the osteomac population by CD14 + . Those cells were selected for sorting. Shown here is a representative FACS run. This FACS protocol was established with n = 3 donors.
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Gating strategy for cell sorting of osteomacs (CD45 + ALP − <t>CD14</t> + ). After Gating of single cells, using the width of the forward and side-scatter signal (FSC-W, SSC-W), living cells were selected (Zombie-Aqua-). CD45 + ALP − cells were then selected to identify the osteomac population by CD14 + . Those cells were selected for sorting. Shown here is a representative FACS run. This FACS protocol was established with n = 3 donors.
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Gating strategy for cell sorting of osteomacs (CD45 + ALP − <t>CD14</t> + ). After Gating of single cells, using the width of the forward and side-scatter signal (FSC-W, SSC-W), living cells were selected (Zombie-Aqua-). CD45 + ALP − cells were then selected to identify the osteomac population by CD14 + . Those cells were selected for sorting. Shown here is a representative FACS run. This FACS protocol was established with n = 3 donors.
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cd14 pe vio770  (Miltenyi Biotec)
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( A ) Using flow cytometric analysis of fixed whole blood, monocytes were identified as <t>CD14</t> + cells within the CD45 + cell population. CD61 + cells within the CD14 + monocyte population were defined as monocyte-platelet aggregates (MPA). ( B ) shows MPA levels from patients with available MPA levels at both time points (TP). High MPA is defined as ≥ 14.1% and low MPA as ≤ 11.7%.
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cd14 anti human  (Miltenyi Biotec)
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( A ) Using flow cytometric analysis of fixed whole blood, monocytes were identified as <t>CD14</t> + cells within the CD45 + cell population. CD61 + cells within the CD14 + monocyte population were defined as monocyte-platelet aggregates (MPA). ( B ) shows MPA levels from patients with available MPA levels at both time points (TP). High MPA is defined as ≥ 14.1% and low MPA as ≤ 11.7%.
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Image Search Results


Gating strategy for cell sorting of osteomacs (CD45 + ALP − CD14 + ). After Gating of single cells, using the width of the forward and side-scatter signal (FSC-W, SSC-W), living cells were selected (Zombie-Aqua-). CD45 + ALP − cells were then selected to identify the osteomac population by CD14 + . Those cells were selected for sorting. Shown here is a representative FACS run. This FACS protocol was established with n = 3 donors.

Journal: Life

Article Title: Isolation of Human Osteal Macrophages

doi: 10.3390/life16030376

Figure Lengend Snippet: Gating strategy for cell sorting of osteomacs (CD45 + ALP − CD14 + ). After Gating of single cells, using the width of the forward and side-scatter signal (FSC-W, SSC-W), living cells were selected (Zombie-Aqua-). CD45 + ALP − cells were then selected to identify the osteomac population by CD14 + . Those cells were selected for sorting. Shown here is a representative FACS run. This FACS protocol was established with n = 3 donors.

Article Snippet: CD14-PE , 130-110-519 , Miltenyi Biotec , 1/50.

Techniques: FACS

Macrophage surface marker expression of osteomacs isolated by MACS ® . The immunofluorescence (IF) images show staining for the macrophage markers CD14, CD45 and markers for M1-like (CD86 and CD68) and M2-like macrophage polarization (CD163, CD209) together with nuclei staining by using DAPI (blue). Arrows indicate thread-like configuration of some osteomacs. Shown here are representative IF images. IF staining was performed with n = 2 donors.

Journal: Life

Article Title: Isolation of Human Osteal Macrophages

doi: 10.3390/life16030376

Figure Lengend Snippet: Macrophage surface marker expression of osteomacs isolated by MACS ® . The immunofluorescence (IF) images show staining for the macrophage markers CD14, CD45 and markers for M1-like (CD86 and CD68) and M2-like macrophage polarization (CD163, CD209) together with nuclei staining by using DAPI (blue). Arrows indicate thread-like configuration of some osteomacs. Shown here are representative IF images. IF staining was performed with n = 2 donors.

Article Snippet: CD14-PE , 130-110-519 , Miltenyi Biotec , 1/50.

Techniques: Marker, Expressing, Isolation, Immunofluorescence, Staining

( A ) Using flow cytometric analysis of fixed whole blood, monocytes were identified as CD14 + cells within the CD45 + cell population. CD61 + cells within the CD14 + monocyte population were defined as monocyte-platelet aggregates (MPA). ( B ) shows MPA levels from patients with available MPA levels at both time points (TP). High MPA is defined as ≥ 14.1% and low MPA as ≤ 11.7%.

Journal: JCI Insight

Article Title: A platelet transcriptomic signature of thromboinflammation predicts cardiovascular risk

doi: 10.1172/jci.insight.195824

Figure Lengend Snippet: ( A ) Using flow cytometric analysis of fixed whole blood, monocytes were identified as CD14 + cells within the CD45 + cell population. CD61 + cells within the CD14 + monocyte population were defined as monocyte-platelet aggregates (MPA). ( B ) shows MPA levels from patients with available MPA levels at both time points (TP). High MPA is defined as ≥ 14.1% and low MPA as ≤ 11.7%.

Article Snippet: Citrate-anticoagulated whole blood was fixed with 1% formalin (Crystalgen catalog CG-190), and stained with CD61 FITC (Agilent DAKO item #F0803), CD86 PE (catalog 555665), CD40 V450 (catalog 561219) (BD), CD14 PE Vio770 (catalog 130-110-521), CD45 VioGreen (catalog 130-110-638), CD16 APC Vio770 (catalog 130-113-390), CD11 APC (catalog 130-110-554), CD162 APC (catalog 130-123-841), and CD142 VioBlue (catalog 130-098-921) (Miltenyi Biotec), followed by lysis of red blood cells.

Techniques: